DAVID S. DOUCHES
Professor

Department of Crop and Soil Sciences
East Lansing, MI 48824

Phone: (517) 355-0271 ext. 1194
Fax: (517) 353-5174
E-mail: douchesd@msu.edu

Joined Department: 1988
Appointment: 90% research 10% teaching

EDUCATION:

  • Post Doctoral Fellow in Molecular Biology, Michigan State University, 1987-1988
  • Ph.D., Genetics, University of California, Davis, 1984-1987
  • M.S., Horticulture and Genetics, North Carolina State University, 1982-1984
  • B.S., Plant Science, Rutgers University (Cook College), 1977-1981

RESEARCH INTERESTS AND SPECIALIZATIONS:

Potato Breeding & Genetics:

Development and utilization of molecular markers (isozyme, RFLP, and RAPD)
Utilization of exotic germplasm
Cytogenetics of 2n gametes
Genetics and breeding for disease (late blight, scab) and insect (Colorado potato beetle and potato tuber moth) resistances
Integration of conventional breeding and genetic engineering (Bt)

 

SELECTED RESEARCH PUBLICATIONS:

  • Coombs, J.J., D. S. Douches, S. G. Cooper, E. J. Grafius, W. L. Pett, and D. D. Moyer.  2005. Combining natural and engineered host plant resistance mechanisms in potato (Solanum tuberosum L.) For Colorado potato beetle (leptinotarsa decemlineata say): choice and no-choice field studies.  J. Am. Soc. Hort.Sci. 130(6):857-864.
     
  • Kirk, W.W., F. M. Abu-El Samen, J.B. Muhinyuza, R. Hammerschmidt, D. S. Douches, C. A. Thill, H.Groza and A. L. Thompson. 2005. Evaluation of Potato Late blight Management Utilizing  SEQ CHAPTER \h \r 1Host Plant Resistance and Reduced Rates and Frequencies of Fungicide Applications. Crop Protection. 24:961-970.
     
  • Douches, D.S., W.W. Kirk, M.A. Bertram, and B.A. Niemira.  2002.  Foliar and tuber assessment of late blight (Phytophthora infestans Mont. deBary) reaction in cultivated potato (Solanum tuberosum L.). Potato Research. 45:215-224. 
     
  • Douches, D.S. and E.J. Grafius. 2005. Transformation for Insect Resistance. In. Genetic Improvement of Solanaceous Crops, Vol. 2: Potato (Eds. M.K. Razdan and A.K. Mattoo), Science Publishers, Inc. USA  pp. 235-266.
     
  • Long, C., Snapp, S.S., D. Douches and R. Chase.  2004. Tuber yield, storability and quality in response to nitrogen fertility and plant population density in four new Michigan cultivars. Amer. J. of Potato Research 81:347-357.
     
  • Coombs, J. J., L.M. Frank, D.S. Douches.  2004.  An applied fingerprinting system for cultivated potato using simple sequence repeats.  Amer. J. Potato Res.  81: 243-250.
     
  • Douches, D.S. J. Coombs, K. Felcher and W. Kirk. 2004.  Foliar reaction to Phytophthora infestans in inoculated potato field trials in Michigan.  Amer. J. Potato Res.  81:443-448.
     
  • Cooper, S. G. D. S. Douches and E.J. Grafius.  2004.  Combining genetic engineering and traditional breeding to provide durable resistance in potatoes to Colorado potato beetle.  Entomologia Experimentalis et Applicata.  112: 37-46.
     
  • Bisognin, D.A. D.S. Douches, L. Buszka, G. Bryan and D. Wang. 2004.  Mapping late blight resistance in Solanum microdontum Bitter.  Crop Science 45: 340-345.
     
  • J.B. Muhinyuza, Kirk, W.W., Hammerschmidt, R. and Douches, D. 2004. Impact of plant age and leaf position on susceptibility to late blight. Proceedings of the Sixth Triennial Conference of the African Potato Association.  314-326.
     
  • J.B. Muhinyuza, Kirk, W.W., Hammerschmidt, R. and Douches, D. 2004.  Combining host plant resistance with managed fungcide applications for control of potato late blight. Proceedings of the Sixth Triennial Conference of the African Potato Association. 329-346.
     
  • Douches, D.S., W. Pett, F. Santos, J. Coombs, E. Grafius, W. Li, E. Anis Metry  , M. Madkour and T. Nasr El-Din. 2004.  Field and Storage Testing Bt-Potatoes for Resistance to Potato Tuber Moth (Lepidoptera: Gelichiidae). J. Econ. Entomol. 97(4):1425-1431.
     
  • Douches,D.S., J. Coombs, K. Jastzrebski, R. Hammerschmidt, W.W. Kirk, C. Long, R.W. Chase and S. Snapp. 2003.  Boulder: A round white multipurpose potato variety.  Amer. J. Potato Res. 80:345-352.
     
  • Felcher, K.J., D.S. Douches, W.W. Kirk, R. Hammerschmidt. 2003. Expression of a fungal glucose oxidase gene in three potato cultivars with different susceptibility to late blight (Phytophthora infestans Mont. deBary).  J. Amer. Soc. Hort. Sci. 128: 238-245.
     
  • Coombs, J.J., D.S. Douches, W. Li, E.J. Grafius, and W.L. Pett. 2003.  Field Evaluation of natural, engineered, and combined resistance mechanisms in potato for control of Colorado potato beetle.  J. Amer. Soc. Hort. Sci. 128(2):219-224.
     
  • Coombs, J.J., D.S. Douches, W. Li, E.J. Grafius, and W.L. Pett.  2002.  Combining engineered (Bt-cry3A) and natural resistance mechanisms in potato (Solanum tuberosum L.) for control of the Colorado potato beetle. J. Amer. Soc. Hort. Sci. 127:62-68.
     
  • Bisognin, D.A. and D.S. Douches, 2002.  Early generation selection for potato tuber quality in progenies of late blight resistant parents. Euphytica 127:1-9.
     
  • Bisognin, D.A., D.S. Douches, K. Jastrzebski and W.W. Kirk. 2002. Half-sib progeny evaluation and selection of potatoes resistant to the US8 genotype of Phytophthora infestans from crosses between resistant and susceptible clones. Euphytica 123:129-138.  
     
  • Bisognin, D. A. and D.S. Douches. 2002.  Genetic diversity in diploid and tetraploid potato late blight resistant germplasm. HortSci. 37:178-183.
     
  • Lagnaoui, A., V. Canedo, and D.S. Douches. 2001.  Evaluation if Bt-cry1Ia1 (cryV) transgenic potatoes on two species of potato tuber moth, Phthorimaea operculella and Symmetrischema tangolias (Lepidoptera: Gelechiidae) in Peru. CIP Program Report 1999-2000. pp. 117-121.
     
  • Douches, D.S., K.J. Felcher, J. Coombs, C. Long, R. Hammerschmidt, W.W. Kirk.  2001.  Michigan Purple:  A Purple-Skinned Tablestock Variety.  Amer. J. Potato Res. 78:421-424.
     
  • Douches, D.S., K. Jastrzebski, J. Coombs, R.W. Chase, R. Hammerschmidt, W.W. Kirk.  2001.  Liberator:  A Round White Chip-processing Variety with Resistance to Scab.  Amer. J. Potato Res. 78:425-431. 
     
  • Douches, D.S. K. Jastrzebski, J. Coombs, W.W. Kirk, K. J. Felcher, R. Hammerschmidt, R.W. Chase.  2001.  Jacqueline Lee:  A Late Blight Resistant Tablestock Variety.  Amer. J. Potato Res. 78:413-419.
     
  • Kirk, W.W., Felcher, K.J., Douches, D.S., Coombs. J., Stein, J.M., Baker, K.M. and Hammerschmidt, R.  2001. Effect of Host Plant Resistance and Reduced Rates and Frequencies of Fungicide Application to Control Potato Late Blight. Plant Disease 85(10): 1113-1118.
     
  •  Kirk, W.W., K. J. Felcher, D. S. Douches, B. A. Niemira and R. Hammerschmidt. 2001.  Susceptibility of Potato (Solanum tuberosum L.) Foliage and Tubers to the US8 Genotype of Phytophthora infestans, Amer. J. Potato Res.78:319-322.
     
  • Douches, D.S.,T.J.  Kisha, W. Li, W.L.  Pett, and E.J. Grafius.  2001. Effectiveness of natural and engineered host plant resistance in potato to the Colorado potato beetle (Leptinotarsa decemlineata (Say) HortSci. 36:967-970.
     
  • Douches, D.S., J.B. Bamberg, W. Kirk, K. Jastrzebski, B.A. Niemira, J. Coombs, D.A. Bisognin, D.A., and K.J. Felcher.  2001. Evaluation of wild Solanum species for resistance to the US-8 genotype of Phytophthora infestans utilizing a fine-screening technique. Amer. J. Potato Res.  78:159-165.
     
  • Kirk, W.W., D.S. Douches, R. Hammerschmidt, J.M. Stein, K. J. Felcher, K. M. Baker and B. A. Niemira. 2001. Combining varietal resistance with managed fungicide applications for the control of potato late blight.  African J. of Crop Science. 5: 329 - 335.
     
  • Pett, W., D. Douches and E. Grafius. 2000.  Insect control:  Durability and breakdown of resistance.  In:  Proceedings of the International Workshop on transgenic potatoes for the benefit of resource-poor farmers in developing countries. International Potato Center, pp.82-86.
     
  • Mohammed, A. D.S. Douches, W. Pett, E. Grafius, J. Coombs, Liswidowati, W. Li, and M.A. Madkour. 2000. Evaluation of potato tuber moth (Lepidoptera: Gelechiidae) resistance in tubers of Bt-cry5 transgenic potato lines. J. Econ. Entomology 93(2):472-476.
     
  • Li, W., K. Zarka, D.S. Douches, J.J. Coombs, W.L. Pett, and E.J. Grafius. 1999. Co-expression of potato PVY0 coat protein gene and cryV-Bt genes in potato (Solanum tuberosum L.). J. Amer. Soc. Hort. Sci. 124(3):218-223.
     
  • Douches, D.S. and D.L. Maas. 1998. Comparison of FDR- and SDR-derived.
     tetraploid progeny from 2x-4x crosses using dihaploids of Solanum tuberosum L. that produce mixed modes of 2n eggs. Theor. Appl. Genet. 97:1307-1313.
     
  • Douches, D.S., A.L. Westedt, K. Zarka, and E.J. Grafius. 1998. Transformation of CryV-Bt transgene combined with natural resistance mechanisms for resistance to tuber moth in potato (Solanum tuberosum L.) HortScience 33(6):1053-1056.
     
  • Haynes, K.G., D.H. Lambert, B.J. Christ, D.P. Weingartner, D.S. Douches, J.E. Backlund, G. Secor, W. Fry, and W. Stevenson. 1998. Phenotypic stability of resistance to late blight in potato clones evaluated at eight sites in the United States. Amer. J. Potato Res 75:211-217.
     
  • Ransom, C.V., D.S. Douches, and J.J. Kells. 1998. Isozyme and RAPD variation among and within hemp dogbane (Apocynum cannabinum) populations. Weed Sci. 46:408-413.
     
  • Ray, H., D.S. Douches, and R. Hammerschmidt. 1998. Transformation of potato with cucumber peroxidase: expression and disease response. Phys. Mol. Plant Path. 53:93-103.
     
  • Warnke, S.E., D.S. Douches, and B.E. Branham. 1998. Isozyme analysis supports allotetraploid inheritance in tetraploid creeping bentgrass (Agrostis palustris Huds.). Crop Science 38:801-805.
     
  • Westedt, A.L., D.S. Douches, W. Pett, and E.J. Grafius. 1998. Evaluation of natural and engineered resistance mechanisms in Solanum tuberosum L. for resistance to Phthorimaea operculella Zeller. J. Econ. Ent. 91(2):552-556.
     
  • Douches, D.S., W.W. Kirk, K. Jastrzebski, C. Long, and R. Hammerschmidt. 1997. Greenhouse screening of potato varieties and advanced breeding lines (Solanum tuberosum L.) for susceptibility to Phytophthora infestans (Mont.) de Bary. Am. Potato J. 74:75-86.
     
  • Schneider, K. and D.S. Douches. 1997. The evaluation of simple sequence repeats (SSRs) to fingerprint North American potato cultivars. Am. Potato J. 74:149-160.
     
  • Warnke, S.E., D.S. Douches, and D.E. Branham. 1997. Relationships among creeping bentgrass (Agrostis palustris Huds.) cultivars based on isozyme polymorphisms. Crop Science 37:203-207.
     
  • Douches, D.S., K. Jastrzebski, D. Maas, and R.W. Chase. 1996. Assessment of potato breeding over the past century. Crop Science 36:1544-1552.
     
  • Spooner, D.M., J. Tivang, J. Nienhuis, J.T. Miller, D.S. Douches, and A. Contreras-M. 1996. Comparison of four molecular markers in measuring relationships among the wild potato relatives Solanum section Etuberosum (subgenus Potato). Theor Appl Genet 92:532-540.
     
  • Sosinski, B. and D.S. Douches. 1996. Use of PCR-based DNA amplification to fingerprint North American potato cultivars. HortScience 31(1):130-133.
  • Douches, D.S. and Freyre, R. 1994. Identification of genetic factors influencing chip color in diploid potato (Solanum spp.). Am. Potato J. 71:581-590.
     
  • Freyre, R. and D.S. Douches. 1994. Quantitative trait loci analysis of specific gravity in diploid potato (Solanum spp.) over environments: Development of a model for marker-assisted selection. Crop Science 34:1361-1368.
     
  • Freyre, R., S. Warnke, B. Sosinski, and D.S. Douches. 1994. Quantitative trait loci analysis of tuber dormancy in diploid potato (Solanum spp.). Theor. Appl. Genet. 89:474-480.
     
  • Freyre, R. and D.S. Douches. 1994. Isoenzymatic identification of quantitative traits in crosses between heterozygous parents: Mapping tuber traits in diploid potato (Solanum ssp.). Theor. Appl. Genet. 87(7):764-772.

 

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