CSS/HRT 451
Cellular and Molecular Principles and Techniques for Plant Sciences

Topics

CSS/HRT 451- Course Schedule--Spring Semester 2004


January 13	Course and laboratory orientation
January 20	Agrobacterium transformation
January 27	Databases
February 3	Genetics of trangenes
February 10	Reporter genes
February 17	Transient expression
February 24	Biolistic method, Genomics Tech Facility
March 2	Midterm I; Plasmid DNA isolation
March 8-12	Spring Break
March 16	Plant DNA isolation/DNA quantification
March 23	DNA digestion/PCR
March 30	Agarose & Polyacrylamide gel electrophoresis of amplified DNA
April 6	Southern transfer/electrophoresis of restricted DNA
April 13	Probe labeling/DNA hybridization
April 20	Southern analysis non-rad detection
April 27	Midterm II; Southern analysis results

UNKNOWN # 1

GACGTCTCGGATGGGACAAGTTTCATGGAATCAGTTCGGGA
GGGAAACCGTTTTTTTGATTCAGGGAGGCATAGGAATTTGGT
GTCCAATGAGAGAATCAATAGAGGTGGTGGAAAGCAAACTA
ATAATGGACGGAAATTTTCTGTACGGTCTGCTATTTTGGCTAC
TCCATCTGGAGAACGGACGATGACATCGGAACAGATGGTCTA
TGATGTGGTTTTGAGGCAGGCAGCCTTGGTGAAGAGGCAACT
GAGATCTACCAATGAGTTAGAAGTGAAGCCGGATATACCTAT
TCCGGGGAATTTGGGCTTGTTGAGTGAAGCATATGATAGGTGT
GGTGAAGTATGTGCAGAGTATGCAAAGACGTTTAACTTAGGA
ACTATGCTAATGACTCCCGAGAGAAGAAGGGCTATCTGGGCA
ATATATGTATGGTGCAGAAGAACAGATGAACTTGTTGATGGCC
CAAACGCATCATATATTACCCCG

UNKNOWN # 2

1 agagagagag aggaaggaaa gattaatcat ggctctcaaa gtgttaagtg ttgcaactca
61 aatggcgatt cctagcaacc taacgacatg tcttcaaccc tcacacttca aatcttctcc
121 aaaactgtta tctagcacta acagtagtag tcggtctcgc ctccgtgtgt attgctcctc
181 ctcgcaactc actactgaaa gacgatccgg aaactacaac ccttctcgtt gggatgtcaa
241 cttcatccaa tcgcttctca gtgactataa ggaggacaaa cacgtgatta gggcttctga
301 gctggtcact ttggtgaaga tggaactgga gaaagaaacg gatcaaattc gacaacttga
361 gttgatcgat gacttgcaga ggatggggct gtccgatcat ttccaaaatg agttcaaaga
421 aatcttgtcc tctatatatc tcgaccatca ctattacaag aacccttttc caaaagaaga
481 aagggatctc tactccacat ctcttgcatt taggctcctc agagaacatg gttttcaagt
541 cgcacaagag gtattcgata gtttcaagaa cgaggagggt gagttcaaag aaagccttag
601 cgacgacacc agaggattgt tgcaactgta tgaagcttcc tttctgttga cggaaggcga
661 aaccacgctc gagtcagcga gggaattcgc caccaaattt ttggaggaaa aagtgaacga
721 gggtggtgtt gatggcgacc ttttaacaag aatcgcatat tctttggaca tccctcttca
781 ttggaggatt aaaaggccaa atgcacctgt gtggatcgaa tggtatagga agaggcccga
841 catgaatcca gtagtgttgg agcttgccat actcgactta aatattgttc aagcacaatt
901 tcaagaagag ctcaaagaat ccttcaggtg gtggagaaat actgggtttg ttgagaagct
961 gcccttcgca agggatagac tggtggaatg ctacttttgg aatactggga tcatcgagcc
1021 acgtcagcat gcaagtgcaa ggataatgat gggcaaagtc aacgctctga ttacggtgat
1081 cgatgatatt tatgatgtct atggcacctt agaagaactc gaacaattca ctgacctcat
1141 tcgaagatgg gatataaact caatcgacca acttcccgat tacatgcaac tgtgctttct
1201 tgcactcaac aacttcgtcg atgatacatc gtacgatgtt atgaaggaga aaggcgtcaa
1261 cgttataccc tacctgcggc aatcgtgggt tgatttggcg gataagtata tggtagaggc
1321 acggtggttc tacggcgggc acaaaccaag tttggaagag tatttggaga actcatggca
1381 gtcgataagt gggccctgta tgttaacgca catattcttc cgagtaacag attcgttcac
1441 aaaggagacc gtcgacagtt tgtacaaata ccacgattta gttcgttggt catccttcgt
1501 tctgcggctt gctgatgatt tgggaacctc ggtggaagag gtgagcagag gggatgtgcc
1561 gaaatcactt cagtgctaca tgagtgacta caatgcatcg gaggcggagg cgcggaagca
1621 cgtgaaatgg ctgatagcgg aggtgtggaa gaagatgaat gcggagaggg tgtcgaagga
1681 ttctccattc ggcaaagatt ttataggatg tgcagttgat ttaggaagga tggcgcagtt
1741 gatgtaccat aatggagatg ggcacggcac acaacaccct attatacatc aacaaatgac
1801 cagaacctta ttcgagccct ttgcatgaga gatgatgacg agccatcgtt tacttactta
1861 aattctacca aagtttttcg aaggcatagt tcgtaatttt tcaagcacca ataaataagg
1921 agaatcggct caaacaaacg tggcatttgc caccacgtga gcacaaggga gagtctgtcg
1981 tcgtttatgg atgaactatt caatttttat gcatgtaata attaagttca agttcaagag
2041 ccttctgcat atttaactat gtatttgaat ttatcgagtg tgattttctg tctttggcaa
2101 catatatttt tgtcatatgt ggcatcttat tatgatatca tacagtgttt atggatgata
2161 tgatactatc

UNKNOWN # 3

1 aaatgtctaa ttaatggttt tgatcttcca accagatctt cttctctatc tcttcgacaa
61 accctttttt ctgggttctt cggagaggtg atttatcacc aaaaaaaaag attttaaaag
121 tctaaatcct tttttctttc tttccttcaa catgccttta ccttggaaga aatctaaatc
181 tagtcggatc tctagattcg tatcagactt tcaacaatct cctaaacatg gtggatctct
241 cgttgttgaa actggtttcc caacttcttt aatcgatctc tttgttaaga atcgcgatcg
301 tctcaaaaaa caatcttcaa aacgtaacaa caagaacaac aaagcccaga ctgaaacgcg
361 tgttttgtct ccacctcctc ctcatatgtc tttgcagcgt cgtatgtctc ttccttccaa
421 gcttgatcca gctttggtta cagaggattc tcctttggtg agaaagatcg aggaaacttc
481 tgttccggag attatccatg aagacgacgg tggtcatcgc ggtggaggag gaggagggtg
541 tgttttgata gtggtggtgt ttaaggtttt tatggtggct gtgcttgcct tgagcacgaa
601 gaagctcgcc gtggggatca cgctctctgc ttttgccctt ctcttcctcg agcttgcggt
661 ggcgcgtgtc ttcacgtact tgaatctctg tcccgacgcg cagattcgga tagattcttt
721 gatcgataag ctcataggga agcgacataa agagaagcta gaagaagaag aagaagaaga
781 atcaacatcg acgcatgaca gaagaaacaa tgtctacttt gaaatcatcg aagaacctag
841 agaggagatt agagtcactc ctcaatcgga ttcgtcgtca ttggagaagg cgaaacttgt
901 ggtggatgag aagaaagaaa tgcagacgat aagagatgtt atgtttaaga ctgagaaaag
961 caaaagcgcg aagctgaaat caaagattgt gaagaagatt gtgccaaaga agctaaggag
1021 ttataagaag aagaagaaga tgaagaacaa agaaaaagaa gaagaagaag ttgaacttga
1081 aactgaagaa gaagggtcta tgacagaagt ttctagcttg tattcagatg agagaatcgg
1141 gagtgaaatc tctgagaggg atgatttaag ttcgaatccg ccatttttag agagttgtac
1201 agaggttgaa gaagaagaag agactggacc aaaagaggat ctaacgaaag tgattggttt
1261 gatagtgatt gttcttgttg gattgctgtg tgggaaagtc tttgctattg gtctgacact
1321 ctcttggtgt ctgattctca gagttttctg ttgcaaatct caaaccagtt tctgaaacag
1381 aggaaaacat gattcagact ttgggttctt ttttttttgt ggaagtccgt ggcagtggtg
1441 ggccttaggg atcgccgtta gttgttgacg gtgacggtgt tgacggtaga gagacggtga
1501 cgggagtgtt gttgttgggg gtggttggca gttcgaagct agacaattgg tttgcttatg
1561 aaactgtgta aatttgtttt gttgttaaca gagatatata tcgtgttatt atgatggtca
1621 aagagtcaaa gttgttgtta tgaagaacca aaaaaaactg agtaatatat tcttaaaaaa
1681 aaaaaaaaaa a